CATECHOLAMINE ASSAY BY RADIOENZYMATIC METHOD - A PROTOCOL

Authors

  • Nasim Karim
  • Joshua R. berlin

Keywords:

catecholamine, radioenz ymaticassay, Catechol-o-methyl transferase (C O M T )

Abstract

Catecholamines are a group o f compounds identified by the catechol nucleus. The significant catecholamines found naturally in the body are epinephrine, nor- epinephrine and dopamine. They are found in various tissues o f the body like brain, adrenals, GIT, blood, urine and cerebrospinal fluid in picogram quantities. Knowledge of catecholamines is im portant in the diagnosis and managem ent o f pheochromocytoma, CNS tumours like neuroblastoma, Diabetes mellitus, hypertension, coronary disease, angina pectoris, myocardial infarction etc. A nalytical techniques for determ ination o f catecholamines have been developed w hich are generally based upon the use o f isolated enzym e catechol-O -m ethyltransferase (COM T) to transfer a radioactive methyl group from S-adenosyl-L-methionine (SAM) to an endogenous catecholamine acceptor m olecule to form a radioactive O-m ethyl catecholam ine derivative. This article gives a basic out line of the catecholam ine assay along w ith a sim plified protocol that can be easily perform ed in the laboratory.

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Published

2008-12-18

How to Cite

Karim, N., & R. berlin, J. (2008). CATECHOLAMINE ASSAY BY RADIOENZYMATIC METHOD - A PROTOCOL. Journal of the Dow University of Health Sciences (JDUHS), 2(3), 121–126. Retrieved from https://jduhs.jduhs.duhs.edu.pk/index.php/jduhs/article/view/1296

Issue

Section

Technique