Tobacco Associated Oral Cancer amongst Pakistani Group

Abstract

Objective: The aim of this study is to evaluate molecular progression of smokeless tobacco lesions to oral cancer among chewers in Pakistani population.
Materials & Methods: This rendomized control experimental study was carried out between September 2011 to August 2012, Department of Biotechnology, University of Karachi. All chemicals and reagents and instrumentation were obtained from the Biotechnology Department. High Speed Centrifugation was performed at the Centralized Science Laboratory, University of Karachi. Two hundred grams of fifty biopsy proven Oral Squamous Cell Carcinomas, were obtained from Jinnah Postgraduate Medical Centre and Civil Hospital Karachi. All samples were obtained directly from surgical specimen, placed in plastic labeled bottles with patient data and stored in normal saline at -20°C. DNA Extraction Kits from FermentasR
were utilized for extraction of genomic DNA, with amplification for Exon-5 of p53 protein was carried out.
Results: Nineteen positive of fifty patients had 12 of 30 male and 7 of 20 females, with a single band and dimmers. Age predilection showed 7 cases from the fourth decade, 2 each from third, fifth, sixth and seventh decades, while one positive from the second decade. Buccal mucosa was most prevalent site with 9 of 19 cases positive. Alveolus and tongue had 4 of 19 each, while lip had 2 case positives. Betel quid was commonly used, as compared to moist snuff.

Conclusion: 186 bp PCR product were sequenced for mutational analysis of p53 in our local community, with no mutations seen.