Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats

Authors

  • Fatima Rehman Department of Anatomy, Liaquat National Hospital and Medical College Karachi, Pakistan.
  • Zia Ul Islam Department of Anatomy, Liaquat National Hospital and Medical College Karachi, Pakistan.
  • Sama ul Haq Department of Anatomy, Fazaia Ruth Pfau Medical College, Karachi, Pakistan
  • Lubna Faisal Department of Anatomy, Liaquat National Hospital and Medical College Karachi, Pakistan.
  • Sadia Rehman Department of Biochemistry, Bahria University Medical & Dental College, Karachi, Pakistan
  • Santosh Kumar Department of Nephrology, Jinnah Post Graduate Medical Center Karachi, Pakistan.

DOI:

https://doi.org/10.36570/jduhs.2020.3.984

Keywords:

Acini, Diameter, Energy drink, Islets of Langerhan’s, Parenchyma

Abstract

Objective: To assess the effects of energy drinks on the micrometric measurements of pancreas of Wistar Albino rats.

Methods: This experimental study was conducted in the Department of Anatomy, in collaboration with the animal house of Basic Medical Sciences Institute (BMSI), Jinnah postgraduate medical center (JPMC), Karachi from September to October 2018. Thirty adult male Wistar Albino rats were equally divided into three groups (Group A as control, while groups B and C were administered 7.5ml and 15ml orally for four weeks, respectively. Pancreas was dissected out at the end of study. After tissue processing, slides were made and stained with hematoxylin and eosin stain (H&E) to observe the morphology and cytoarchitecture of the tissue.

Results: The findings of the study revealed that the mean diameter of pancreatic acini and islets were significantly lower in Group B (12.70 +- 1.20), (63.52 +- 8.98) as compared to Group A (26.29 +- 4.37), (123.66 +- 22.08) respectively, p-value <0.05. Diameter of pancreatic acini and islets were significantly less in Group C (10.06 +- 0.89), (44.58 +- 7.66) as compared to both Groups A (26.29 +- 4.37), (123.66 +- 22.08) and Group B (12.70 +- 1.20), (63.52 +- 8.98) respectively, p-value <0.01. The parenchyma of the observed organ was also damaged with loss of normal cytoarchitecture as observed on microscopy.

Conclusion: The results obtained in the current study suggest that consumption of energy drinks seriously damages the regular anatomy of the pancreas of Wistar Albino rats.

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Published

2020-12-17

How to Cite

Rehman, F., Islam, Z. U., Haq, S. ul, Faisal, L., Rehman, S., & Kumar, S. (2020). Morphometric Study on the Energy Drink Induced Pancreatitis on Wistar Albino Rats. Journal of the Dow University of Health Sciences (JDUHS), 14(3), 102–106. https://doi.org/10.36570/jduhs.2020.3.984

Issue

Vol. 14 No. 3

Section

Original Articles

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Copyright (c) 2020 Fatima Rehman, Zia Ul Islam, Sama ul Haq, Lubna Faisal, Sadia Rehman, Santosh Kumar

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Articles published in the Journal of Dow University of Health Sciences are distributed under the terms of the Creative Commons Attribution Non-Commercial License https://creativecommons.org/ licenses/by-nc/4.0/. This license permits use, distribution and reproduction in any medium; provided the original work is properly cited and initial publication in this journal.